Cysteine proteinases of microorganisms and viruses
نویسندگان
چکیده
منابع مشابه
Cysteine proteinases and the pathogenesis of amebiasis.
Amebiasis is a major cause of morbidity and mortality throughout the tropical world. Entamoeba histolytica is now recognized as a separate species from the morphologically identical E. dispar, which cannot invade. Cysteine proteinases are a key virulence factor of E. histolytica and play a role in intestinal invasion by degrading the extracellular matrix and circumventing the host immune respon...
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Catalytic efficiencies of proteinase K and mesentericopeptidase were determined using series of peptide-4-nitroanilide substrates and compared with those of subtilisin DY, savinase and esperase. For each enzyme the subsites S1-S4 were characterized. The data for the enzyme specificities were related to our high resolution X-ray models of the five enzymes and their complexes with peptides. The c...
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NADH could not be freeze-stored at -25°C in 50 mMsodium phosphate buffer (pH range 6-8 20°C) without serious loss of the nucleotide; however, no losses were seen upon freeze storage in 50 mM-Hepes buffer (pH range 7-8, 20°C). The rate of NADH degradation was greater (approx. 2-fold) in 50 mMthan in 5 mM-sodium phosphate buffer, although NADH values at both buffer concentrations were similar (5%...
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Cysteine proteinases also referred to as thiol proteases play an essential role in plant growth and development but also in senescence and programmed cell death, in accumulation of storage proteins such as in seeds, but also in storage protein mobilization. Thus, they participate in both anabolic and catabolic processes. In addition, they are involved in signalling pathways and in the response ...
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A synthesis of peptidylfluoromethanes is described that utilizes the conversion of phthaloyl amino acids into their fluoromethane derivatives. These can be deblocked and elongated. The inactivation of chymotrypsin by Cbz-Phe-CH2F (benzyloxycarbonylphenylalanylfluoromethane) was found to be considerably slower than that of the analogous chloromethane. The fluoromethane analogue inactivates chymo...
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ژورنال
عنوان ژورنال: Biochemistry (Moscow)
سال: 2008
ISSN: 0006-2979,1608-3040
DOI: 10.1134/s000629790801001x